SPATS2L Polyklonaler Antikörper

SPATS2L Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29930-1-PBS

Synonyme

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  WB analysis using 29930-1-AP (same clone as 29930-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29930-1-AP (SPATS2L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

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  WB analysis using 29930-1-AP (same clone as 29930-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29930-1-AP (SPATS2L antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 29930-1-AP (same clone as 29930-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29930-1-AP (SPATS2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 29930-1-AP (same clone as 29930-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29930-1-AP (SPATS2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 29930-1-AP (same clone as 29930-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29930-1-AP (SPATS2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 29930-1-AP (same clone as 29930-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29930-1-AP (SPATS2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 29930-1-AP (same clone as 29930-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SPATS2L antibody (29930-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 29930-1-AP (same clone as 29930-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SPATS2L antibody (29930-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 29930-1-AP (same clone as 29930-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SPATS2L antibody (29930-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29930-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA SPATS2L und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPATS2L fusion protein Ag31157
• Vollständiger Name: viral DNA polymerase-transactivated protein 6
• Berechnetes Molekulargewicht: 488 aa, 54 kDa
• Beobachtetes Molekulargewicht: 54-65 kDa
• GenBank-Zugangsnummer: BC018736
• Gene symbol: SPATS2L
• Gene ID (NCBI): 26010
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPATS2L encodes spermatogenesis associated and serine-rich 2-like protein, also named DNAPTP6 (DNA polymerase transactivated protein 6) in human (PMID:11230166). SPATS2L acts as an important regulator of β2-adrenergic receptor down-regulation (PubMed: 25562107). MiR-1269a down-regulates the expression of SPATS2L and LRP6, thereby inhibiting the proliferation of liver cancer cells (PMID: 28081866, PMID:35252180). SPATS2L has several isoforms by alternative splicing.