SPEG Polyklonaler Antikörper

SPEG Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18716-1-AP

Synonyme

APEG 1, APEG1, BPEG, KIAA1297, SPEG, SPEG complex locus, SPEGalpha, SPEGbeta

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  IHC staining of mouse heart using 18716-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18716-1-AP (SPEG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 18716-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18716-1-AP (SPEG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 18716-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18716-1-AP (SPEG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 18716-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18716-1-AP (SPEG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

18716-1-AP bindet in IHC, ELISA SPEG und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: SPEG complex locus
• Berechnetes Molekulargewicht: 354 kDa
• GenBank-Zugangsnummer: NM_005876
• Gene symbol: SPEG
• Gene ID (NCBI): 10290
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPEG, also named as BPEG, APEG1 and KIAA1297, is highly similar to the corresponding rat and mouse proteins. Expression of SPEG is thought to serve as a marker for differentiated vascular smooth muscle cells which may have a role in regulating growth and differentiation of this cell type. SPEG is expressed in vascular smooth muscle cells and is down-regulated by vascular injury. Multiple transcripts are produced from the SPEG complex locus by alternative splicing and alternative promoter usage. The antibody recognizes the C-term of SPEG.