SPERT Polyklonaler Antikörper

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SPERT Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17826-1-PBS

Synonyme

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  WB analysis of mouse testis using 17826-1-AP (same clone as 17826-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17826-1-AP (SPERT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17826-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 17826-1-AP (same clone as 17826-1-PBS)

  WB result of SPERT antibody (17826-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPERT transfected PC-3 cells. This data was developed using the same antibody clone with 17826-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 17826-1-AP (same clone as 17826-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 17826-1-AP (SPERT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17826-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse spermatophore using 17826-1-AP (same clone as 17826-1-PBS)

  mouse spermatophore tissue were subjected to SDS PAGE followed by western blot with 17826-1-AP (SPERT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17826-1-PBS in a different storage buffer formulation.

• 

  IF Staining of PC-3 using 17826-1-AP (same clone as 17826-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using SPERT antibody (17826-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17826-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17826-1-PBS bindet in WB, IF/ICC, Indirect ELISA SPERT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPERT fusion protein Ag12200
• Vollständiger Name: spermatid associated
• Berechnetes Molekulargewicht: 448 aa, 47 kDa, 52 kDa
• Beobachtetes Molekulargewicht: 48-52 kDa
• GenBank-Zugangsnummer: BC027609
• Gene symbol: SPERT
• Gene ID (NCBI): 220082
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPERT (spermatid associated protein), also known as CBY2 (chibby homolog 2) or NURIT, is a novel leucine-zipper protein belonging to the chibby family of proteins [PMID:12204287]. Uniquely expressed in the spermatid flower-like structure, SPERT interacts with Nek1, a member of the NIMA-family kinase family that is associated centrosomal stability and ciliogenesis. It contains a leucine-zipper motif and two coiled-coil regions, and is transcribed through the elongation stage of the spermatids. SPERT is absent from mature spermatozoa and is thought to be involved in transporting proteins that are to be discarded via the residual bodies. [PMID:12204287]