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SPG7 Polyklonaler Antikörper

SPG7 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27801-1-PBS

Synonyme

CAR, CMAR, EC:3.4.24.-, Mitochondrial inner membrane m-AAA protease component paraplegin, Paraplegin

Filter:
  • Western Blot (WB) analysis of various lysates using SPG7 Polyclonal antibody (27801-1-AP)

    WB analysis using 27801-1-AP (same clone as 27801-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27801-1-AP (SPG7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27801-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using SPG7 Polyclonal antibody (27801-1-AP)

    IP experiment of HeLa using 27801-1-AP (same clone as 27801-1-PBS)

    IP result of anti-SPG7 (IP:27801-1-AP, 4ug; Detection:27801-1-AP 1:500) with HeLa cells lysate 2800 ug. This data was developed using the same antibody clone with 27801-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SPG7 Polyclonal antibody (27801-1-AP)

    IHC staining of human liver using 27801-1-AP (same clone as 27801-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27801-1-AP (SPG7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27801-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SPG7 Polyclonal antibody (27801-1-AP)

    IHC staining of human liver using 27801-1-AP (same clone as 27801-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27801-1-AP (SPG7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27801-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

27801-1-PBS bindet in WB, IHC, IP, Indirect ELISA SPG7 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen SPG7 fusion protein Ag26265
Vollständiger Name spastic paraplegia 7 (pure and complicated autosomal recessive)
Berechnetes Molekulargewicht 88 kDa
Beobachtetes Molekulargewicht54 kDa, 70 kDa, 80kDa
GenBank-ZugangsnummerBC007692
Gene symbol SPG7
Gene ID (NCBI) 6687
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPG7 encodes a nuclear-encoded mitochondrial metalloprotease protein that is a member of the AAA protein family. Two transcript variants encoding distinct isoforms have been identified. The molecular weight of SPG7 is about 88-100 kDa (precursor),80 kDa (intermediate) and 70 kDa (mature)(PMID: 24767997), while the molecular weight of isoform 2 is about 54 kDa.