SPHK1 Rekombinanter Antikörper

SPHK1 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2K12

Kat-Nr. : 82609-1-RR

Synonyme

Sphingosine kinase 1, SK1, EC:2.7.1.91, EC:2.3.1.-, Acetyltransferase SPHK1

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  WB analysis using 82609-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82609-1-RR (SPHK1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 82609-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SPHK1 antibody (82609-1-RR, Clone: 2K12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  Affinity and Kinetic Characterization of 82609-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82609-1-RR against Human SPHK1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, L02-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82609-1-RR bindet in WB, IF/ICC, ELISA SPHK1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SPHK1 fusion protein Ag1051
• Vollständiger Name: sphingosine kinase 1
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC008040
• Gene symbol: SPHK1
• Gene ID (NCBI): 8877
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPHK(Sphingosine kinase 1) is also named as SPHK, SPK.It catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions and is precariously perched at the balance point between progrowth and pro-death signalling in the cell.Endogenous SPHK1 purified from placenta has identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties(PMID:10947957).It has 2 isoforms produced by alternative splicing and can form the oligomeric structure(PMID:22335554).