SPI1 Monoklonaler Antikörper

SPI1 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2H3D3

Kat-Nr. : 66618-2-PBS

Synonyme

2H3D3, 31 kDa transforming protein, 31 kDa-transforming protein, OF, PU.1

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  WB analysis of Daudi using 66618-2-Ig (same clone as 66618-2-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66618-2-Ig (same clone as 66618-2-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

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  WB analysis of rat spleen using 66618-2-Ig (same clone as 66618-2-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 66618-2-Ig (same clone as 66618-2-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66618-2-Ig (SPI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 66618-2-Ig (same clone as 66618-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

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  IHC staining of Human tonsillitis using 66618-2-Ig (same clone as 66618-2-PBS)

  Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:4000 (under 20x lens). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

• 

  IHC staining of Human tonsillitis using 66618-2-Ig (same clone as 66618-2-PBS)

  Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:4000 (under 20x lens). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

• 

  IHC staining of Human tonsillitis using 66618-2-Ig (same clone as 66618-2-PBS)

  Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:2000 (under 20x lens). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 66618-2-Ig (same clone as 66618-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 66618-2-Ig (SPI1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66618-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66618-2-PBS bindet in WB, IHC, Indirect ELISA SPI1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: spleen focus forming virus (SFFV) proviral integration oncogene spi1
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 32-38 kDa
• GenBank-Zugangsnummer: NM_003120
• Gene symbol: SPI1
• Gene ID (NCBI): 6688
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPI1, also named as 31 kDa-transforming protein, is a 270 amino acid protein, which belongs to the ETS family. SPI1 is highly expressed in both FV-P and FV-A-induced erythro-leukemia cell lines that have undergone rearrangements of the SPI1 gene due to the insertion of SFFV.