SPINT3 Polyklonaler Antikörper

SPINT3 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31931-1-AP

Synonyme

HKIB9, Kunitz-type protease inhibitor 3

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  WB analysis using 31931-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31931-1-AP (SPINT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 31931-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31931-1-AP (SPINT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 31931-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using SPINT3 antibody (31931-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A549-Zellen, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

31931-1-AP bindet in WB, IF/ICC, ELISA SPINT3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPINT3 fusion protein Ag35289
• Vollständiger Name: serine peptidase inhibitor, Kunitz type, 3
• Berechnetes Molekulargewicht: 10 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: NM_006652.1
• Gene symbol: SPINT3
• Gene ID (NCBI): 10816
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Serine peptidase inhibitor, Kunitz type 3 (SPINT3, also known as HKIB9) might be related to serine-type endopeptidase inhibitor activity, receptor antagonist activity, and transforming growth factor beta binding (PMID: 21988899). The calculated molecular weight of SPINT3 is 10 kDa, which is lower than its observed molecular weight of 30-35 kDa by SDS-PAGE detection (PMID: 21988899).