PBK/SPK Polyklonaler Antikörper

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• KD/KO Validated

PBK/SPK Polyklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 16110-1-AP

Synonyme

PBK, Cancer/testis antigen 84, CT84, EC:2.7.12.2, Lymphokine-activated killer T-cell-originated protein kinase

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  WB analysis using 16110-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16110-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 16110-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 16110-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16110-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 16110-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16110-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 16110-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16110-1-AP (PBK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 16110-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16110-1-AP (PBK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 16110-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16110-1-AP (PBK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 16110-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16110-1-AP (PBK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 16110-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16110-1-AP (PBK antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin cancer using 16110-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16110-1-AP (PBK antibody at dilution of 1:50 (under 40x lens).

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  FC experiment of HepG2 using 16110-1-AP

  (blue)1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Anti-Human PBK (16110-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) at dilution 1:1000(red), or 0.8 ug rabbit IgG isotype control. Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, Maus-Eierstockgewebe, Maushodengewebe, NIH/3T3-Zellen, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Lungenkarzinomgewebe, humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:3000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

16110-1-AP bindet in WB, IHC, IF, FC (Intra), IP, CoIP, ELISA PBK/SPK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PBK/SPK fusion protein Ag9033
• Vollständiger Name: PDZ binding kinase
• Berechnetes Molekulargewicht: 322 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36-40 kDa
• GenBank-Zugangsnummer: BC015191
• Gene symbol: PBK
• Gene ID (NCBI): 55872
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PBK(PDZ-binding kinase) is also named as TOPK, CT84, Nori-3, SPK and belongs to the MAP kinase kinase subfamily. PBK may have a role in the regulation of cellular proliferation and progression of the cell cycle. It has a characteristic cdc2ycyclin B phosphorylation site(SyT-P-X-KyR) at its N terminus,which is conserved across species, and it is phosphorylated in a cell cycle-dependent manner at mitosis, and that this phosphorylation is required for its activation(PMID:10779557).

Publikationen

Species	Application	Title
human	WB	Genomics Proteomics Bioinformatics; Specific Regulation of m6A by SRSF7 Promotes the Progression of Glioblastoma.; Authors - Yixian Cun
mouse	WB,IF	FASEB J; Autophagy plays a positive role in induction of epidermal proliferation.; Authors - Junyi Wang
mouse	IF	Invest Ophthalmol Vis Sci; Single-Cell Transcriptomics Reveals Cellular Heterogeneity and Complex Cell-Cell Communication Networks in the Mouse Cornea; Authors - Yueh-Feng Wu
human	IHC	Biomolecules; Identification of a Fibroblast-Related Prognostic Model in Glioma Based on Bioinformatics Methods; Authors - Haofuzi Zhang
mouse	WB,IF	Invest Ophthalmol Vis Sci; Single-Cell RNA Transcriptome Helps Define the Limbal/Corneal Epithelial Stem/Early Transit Amplifying Cells and How Autophagy Affects This Population.; Authors - Nihal Kaplan ; KD Validated
rat	WB	Front Endocrinol (Lausanne); PBK/TOPK Inhibitor Suppresses the Progression of Prolactinomas.; Authors - Kejing Zhu