PBK/SPK Rekombinanter Antikörper

PBK/SPK Rekombinant Antikörper für WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242545A11

Kat-Nr. : 84970-1-PBS

Synonyme

PBK, 242545A11, Cancer/testis antigen 84, CT84, EC:2.7.12.2

---

• 

  WB analysis using 84970-1-RR (same clone as 84970-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84970-1-RR (PBK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84970-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP01693-1

  Sandwich ELISA standard curve of MP01693-1, Human PBK Recombinant Matched Antibody Pair - PBS only. 84970-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag9033. 84970-4-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL.

• 

  Sample test of MP01693-1

  The mean PBK concentration was determined to be 342.86 ng/mL in HeLa cell extract based on a 1.30 mg/mL extract load and 1,243.51 ng/mL in Jurkat cell extract based on a 2.00 mg/mL extract load.

• 

  IF Staining of HeLa using 84970-1-RR (same clone as 84970-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PBK/SPK antibody (84970-1-RR, Clone: 242545A11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84970-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 84970-1-RR (same clone as 84970-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PBK/SPK Recombinant antibody (84970-1-RR, Clone:242545A11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84970-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01693-1

  Cytometric bead array standard curve of MP01693-1, PBK Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84970-1-PBS. Detection antibody: 84970-4-PBS. Standard: Ag9033. Range: 0.781-100 ng/mL.

---

Geprüfte Anwendungen

Produktinformation

84970-1-PBS bindet in WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test PBK/SPK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PBK/SPK fusion protein Ag9033
• Vollständiger Name: PDZ binding kinase
• Berechnetes Molekulargewicht: 322 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36-40 kDa
• GenBank-Zugangsnummer: BC015191
• Gene symbol: PBK
• Gene ID (NCBI): 55872
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PBK(PDZ-binding kinase) is also named as TOPK, CT84, Nori-3, SPK and belongs to the MAP kinase kinase subfamily. PBK may have a role in the regulation of cellular proliferation and progression of the cell cycle. It has a characteristic cdc2ycyclin B phosphorylation site(SyT-P-X-KyR) at its N terminus,which is conserved across species, and it is phosphorylated in a cell cycle-dependent manner at mitosis, and that this phosphorylation is required for its activation(PMID:10779557).