SPOCD1 Polyklonaler Antikörper

SPOCD1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 22243-1-AP

Synonyme

RP11 84A19.1, SPOC domain containing 1, SPOCD1

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  WB analysis of SH-SY5Y using 22243-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22243-1-AP (SPOCD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 22243-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 22243-1-AP (SPOCD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22243-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22243-1-AP (SPOCD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, HEK-293-Zellen, humanes Hodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

22243-1-AP bindet in WB, IF, ELISA SPOCD1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPOCD1 fusion protein Ag17734
• Vollständiger Name: SPOC domain containing 1
• Berechnetes Molekulargewicht: 1203 aa, 129 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC114430
• Gene symbol: SPOCD1
• Gene ID (NCBI): 90853
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The PIWI protein MIWI2 (PIWIL4; 610315) and its associated PIWI-interacting RNAs (piRNAs) direct DNA methylation of transposable elements in the male germline. SPOCD1 is an essential nuclear effector of MIWI2- and piRNA-directed DNA methylation (PubMed: 32674113).

Publikationen

Species	Application	Title
human	WB	Oncotarget; Bromodomain protein BRD4 inhibitor JQ1 regulates potential prognostic molecules in advanced renal cell carcinoma.; Authors - Takashi Sakaguchi
human	WB,IF	Cell Death Discov; IFNγ regulates ferroptosis in KFs by inhibiting the expression of SPOCD1 through DNMT3A; Authors - Xiuxia Wang