SPON2 Monoklonaler Antikörper

SPON2 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1H4B11

Kat-Nr. : 66859-1-PBS

Synonyme

1H4B11, Differentially expressed in cancerous and non-cancerous lung cells 1, DIL 1, DIL1, DIL-1

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  WB analysis of LNCaP using 66859-1-Ig (same clone as 66859-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66859-1-Ig (SPON2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66859-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66859-1-Ig (same clone as 66859-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66859-1-Ig (SPON2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66859-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66859-1-Ig (same clone as 66859-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66859-1-Ig (SPON2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66859-1-PBS in a different storage buffer formulation.

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  IF Staining of LNCaP using 66859-1-Ig (same clone as 66859-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using SPON2 antibody (66859-1-Ig, Clone: 1H4B11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66859-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66859-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA SPON2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SPON2 fusion protein Ag14337
• Vollständiger Name: spondin 2, extracellular matrix protein
• Berechnetes Molekulargewicht: 331 aa, 36 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC002707
• Gene symbol: SPON2
• Gene ID (NCBI): 10417
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPON2 (Spondin 2), also known as Mindin, is a member of the F-spondin family of secreted extracellular matrix proteins. This protein has been implicated in axon guidance, immune response and adhesion. SPON2 is essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens (PMID: 14691481). It also functions as an integrin ligand for inflammatory cell recruitment and T-cell priming (PMID: 19153605). Besides, SPON2 has been reported as a prognostic biomarker for ovarian and prostate cancer (PMID: 17490732; 22615945; 23665271).