SPTAN1 Polyklonaler Antikörper

SPTAN1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31676-1-PBS

Synonyme

SPTA2, NEAS, Alpha II spectrin

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  WB analysis using 31676-1-AP (same clone as 31676-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31676-1-AP (SPTAN1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31676-1-PBS in a different storage buffer formulation.

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  WB analysis using 31676-1-AP (same clone as 31676-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31676-1-AP (SPTAN1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31676-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 31676-1-AP (same clone as 31676-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31676-1-AP (SPTAN1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31676-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 31676-1-AP (same clone as 31676-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31676-1-AP (SPTAN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31676-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 31676-1-AP (same clone as 31676-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31676-1-AP (SPTAN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31676-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 31676-1-AP (same clone as 31676-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SPTAN1 antibody (31676-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 31676-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

31676-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA SPTAN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPTAN1 fusion protein Ag36472
• Vollständiger Name: spectrin, alpha, non-erythrocytic 1 (alpha-fodrin)
• Berechnetes Molekulargewicht: 285kDa，2472aa
• Beobachtetes Molekulargewicht: 285 kDa,150 kDa
• GenBank-Zugangsnummer: NM_003127.3
• Gene symbol: SPTAN1
• Gene ID (NCBI): 6709
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPTAN1 (Nonerythroid spectrin αII), also termed α-Fodrin, is a cytoskeletal protein that belongs to the family of spectrins. Spectrins includes several structural proteins (α- and β-spectrin, α-actinin, dystrophin, and utrophin) that build and stabilize the cytoskeleton by forming a hexagonal mesh under the plasma membrane and ensuring stability and organization of organelles in the cell. SPTAN1 encodes for a 2,472 amino acid protein with a predicted molecular weight of 284 kDa and can be cleaved by calpain leading to a 150 kDa fragment (PMID: 31186638).