SPTAN1 Rekombinanter Antikörper

SPTAN1 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242104B7

Kat-Nr. : 84761-2-PBS

Synonyme

242104B7, Alpha II spectrin, NEAS, SPTA2

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  WB analysis using 84761-2-RR (same clone as 84761-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84761-2-RR (SPTAN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84761-2-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 84761-2-RR (same clone as 84761-2-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 84761-2-RR (SPTAN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84761-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 84761-2-RR (same clone as 84761-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84761-2-RR (SPTAN1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84761-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 84761-2-RR (same clone as 84761-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84761-2-RR (SPTAN1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84761-2-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 84761-2-RR (same clone as 84761-2-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84761-2-RR (SPTAN1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84761-2-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 84761-2-RR (same clone as 84761-2-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84761-2-RR (SPTAN1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84761-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84761-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84761-2-RR against Human SPTAN1 were performed. The affinity constant is 2.59 nM.

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Geprüfte Anwendungen

Produktinformation

84761-2-PBS bindet in WB, IHC, Indirect ELISA SPTAN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SPTAN1 fusion protein Ag36472
• Vollständiger Name: spectrin, alpha, non-erythrocytic 1 (alpha-fodrin)
• Berechnetes Molekulargewicht: 285kDa，2472aa
• Beobachtetes Molekulargewicht: 285 kDa,150 kDa
• GenBank-Zugangsnummer: NM_003127.3
• Gene symbol: SPTAN1
• Gene ID (NCBI): 6709
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPTAN1 (Nonerythroid spectrin αII), also termed α-Fodrin, is a cytoskeletal protein that belongs to the family of spectrins. Spectrins includes several structural proteins (α- and β-spectrin, α-actinin, dystrophin, and utrophin) that build and stabilize the cytoskeleton by forming a hexagonal mesh under the plasma membrane and ensuring stability and organization of organelles in the cell. SPTAN1 encodes for a 2,472 amino acid protein with a predicted molecular weight of 284 kDa and can be cleaved by calpain leading to a 150 kDa fragment (PMID: 31186638).