SPTLC1 Monoklonaler Antikörper

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• KD/KO Validated

SPTLC1 Monoklonal Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2D11D7

Kat-Nr. : 66899-1-PBS

Synonyme

Serine-palmitoyl-CoA transferase 1, LCB1, LCB 1, HSAN1, EC:2.3.1.50

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  WB analysis using 66899-1-Ig (same clone as 66899-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66899-1-Ig (SPTLC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66899-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66899-1-Ig (same clone as 66899-1-PBS)

  WB result of SPTLC1 antibody (66899-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPTLC1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66899-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66899-1-Ig (same clone as 66899-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66899-1-Ig (SPTLC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66899-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66899-1-Ig (same clone as 66899-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66899-1-Ig (SPTLC1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66899-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66899-1-Ig (same clone as 66899-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66899-1-Ig (SPTLC1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66899-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66899-1-Ig (same clone as 66899-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SPTLC1 antibody (66899-1-Ig, Clone: 2D11D7 ) at dilution of 1:981 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66899-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50865-2

  Cytometric bead array standard curve of MP50865-2, SPTLC1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66899-4-PBS. Detection antibody: 66899-1-PBS. Standard:Ag7876. Range: 0.195-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

66899-1-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA SPTLC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SPTLC1 fusion protein Ag7876
• Vollständiger Name: serine palmitoyltransferase, long chain base subunit 1
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC007085
• Gene symbol: SPTLC1
• Gene ID (NCBI): 10558
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPTLC1 is a subunit of serine palmitoyltransferase (SPT) which is the key enzyme in sphingolipid biosynthesis and is essential for embryogenesis and cell survival. Mutations in the SPTLC1 gene (C133W, C133Y, V144D, and G387A) were reported to be responsible for the development of an inherited sensory neuropathy (hereditary sensory neuropathy type I, HSN1).