SQRDL Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SQRDL Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(30)

Kat-Nr. : 17256-1-AP

Synonyme

CGI 44, CGI-44, EC:1.8.5.8, SQOR, Sulfide dehydrogenase-like

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  WB analysis using 17256-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17256-1-AP (SQRDL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17256-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17256-1-AP (SQRDL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17256-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17256-1-AP (SQRDL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17256-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17256-1-AP (SQRDL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17256-1-AP

  IP result of anti-SQRDL (IP:17256-1-AP, 4ug; Detection:17256-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human colon cancer using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17256-1-AP (SQRDL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17256-1-AP (SQRDL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17256-1-AP (SQRDL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 17256-1-AP (SQRDL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17256-1-AP (SQRDL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG-Zellen, HepG2-Zellen, HT-29-Zellen, THP-1-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Hodengewebe, Mauslebergewebe, Rattenlebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 25 publications below
• IHC: See 5 publications below

Produktinformation

17256-1-AP bindet in WB, IHC, IP, ELISA SQRDL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SQRDL fusion protein Ag10762
• Vollständiger Name: sulfide quinone reductase-like (yeast)
• Berechnetes Molekulargewicht: 450 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC016836
• Gene symbol: SQRDL
• Gene ID (NCBI): 58472
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SQRDL(Sulfide:quinone oxidoreductase, mitochondrial) catalyzes the oxidation of hydrogen sulfide, with the help of a quinone.Although the SQRDL precursor protein displays a molecular weight of 50 kDa,a prominent band at about 46 kDa is detected by western blot. This discrepancy reflects the cleavage of an N-terminal targeting sequence of 4 kDa during the import of the protein into the mitochondria(PMID:22067608).SQRDL is one of the enzymes involved in H2S signaling in a discrete population of neurons, oligodendrocytes, and endothelial cells.

Publikationen

Species	Application	Title
mouse	WB	Cell; Endogenous Hydrogen Sulfide Production Is Essential for Dietary Restriction Benefits.; Authors - Christopher Hine ; KD Validated
rat,mouse	WB	Nat Commun; Sulfide catabolism ameliorates hypoxic brain injury.; Authors - Eizo Marutani ; KD Validated
mouse	WB,IHC	Redox Biol; Sulfide:quinone oxidoreductase alleviates ferroptosis in acute kidney injury via ameliorating mitochondrial dysfunction of renal tubular epithelial cells; Authors - Fangfang Cai
mouse	WB	EMBO Mol Med; β-RA reduces DMQ/CoQ ratio and rescues the encephalopathic phenotype in Coq9 R239X mice.; Authors - Agustín Hidalgo-Gutiérrez
human	WB	EMBO Mol Med; Coenzyme Q deficiency causes impairment of the sulfide oxidation pathway.; Authors - Marcello Ziosi
mouse	WB	EMBO Mol Med; CoQ deficiency causes disruption of mitochondrial sulfide oxidation, a new pathomechanism associated with this syndrome.; Authors - Marta Luna-Sánchez