FDFT1 Rekombinanter Antikörper

FDFT1 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

230362C10

Kat-Nr. : 83020-1-RR

Synonyme

230362C10, DGPT, EC:2.5.1.21, ERG9, Farnesyl-diphosphate farnesyltransferase

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  WB analysis using 83020-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83020-1-RR (FDFT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 83020-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83020-1-RR (FDFT1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 83020-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FDFT1 antibody (83020-1-RR, Clone: 230362C10 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of L02 using 83020-1-RR

  Immunofluorescent analysis of (4% PFA) fixed L02 cells using FDFT1 antibody (83020-1-RR, Clone: 230362C10 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  FC experiment of U2OS using 83020-1-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human FDFT1 (83020-1-RR, Clone:230362C10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83020-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83020-1-RR against Human FDFT1 were performed. The affinity constant is 0.126 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, HepG2-Zellen, Maushodengewebe, SH-SY5Y-Zellen, T-47D-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: L02-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83020-1-RR bindet in WB, IHC, IF/ICC, FC (Intra), ELISA FDFT1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FDFT1 fusion protein Ag3743
• Vollständiger Name: farnesyl-diphosphate farnesyltransferase 1
• Berechnetes Molekulargewicht: 417 aa, 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC029641
• Gene symbol: FDFT1
• Gene ID (NCBI): 2222
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SQS (Squalene synthase), also known as Farnesyl-diphosphate farnesyltransferase 1 (FDFT1) is a gene that encodes the membrane-associated enzyme squalene synthase, which is the first specific enzyme in cholesterol biosynthesis. FDFT1 is highly expressed in liver, lung, prostate, breast, ovary, bladder, cervix, thyroid, and esophageal cancers, while in colorectal, colon, testicular, uterine, pancreas, and kidney tumors, its expression is downregulated (PMID: 35093030). FDFT1 is a key downstream target of the fasting response and may be involved in CRC cell glucose metabolism (PMID: 32313017).