FDFT1 Rekombinanter Antikörper

FDFT1 Rekombinant Antikörper für IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

230362F3

Kat-Nr. : 83020-2-PBS

Synonyme

230362F3, DGPT, EC:2.5.1.21, ERG9, Farnesyl-diphosphate farnesyltransferase

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  Sandwich ELISA standard curve of MP00097-1

  Sandwich ELISA standard curve of MP00097-1, Human FDFT1 Recombinant Matched Antibody Pair - PBS only. 83020-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3743. 83020-4-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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  Sample test of MP00097-1

  Serum of eight individual healthy human donors was measured. The FDFT1 concentration of detected samples was determined to be 1,817 pg/mL with a range of 368 - 5,103 pg/mL.

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  Sample test of MP00097-1

  The mean FDFT1 concentration was determined to be 8.7 ng/mL in HeLa cell extract based on a 1.5 mg/mL extract load.

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  IF Staining of HepG2 using 83020-2-RR (same clone as 83020-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FDFT1 antibody (83020-2-RR, Clone: 230362F3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83020-2-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 83020-2-RR (same clone as 83020-2-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug FDFT1 Recombinant antibody (83020-2-RR, Clone:230362F3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83020-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00097-1

  Cytometric bead array standard curve of MP00097-1, SQS/FDFT1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83020-2-PBS. Detection antibody: 83020-4-PBS. Standard: Ag3743. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 83020-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 83020-2-PBS against Human FDFT1 was performed. The affinity constant is below 1 pM.

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  Affinity and Kinetic Characterization of 83020-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83020-2-RR against Human FDFT1 were performed. The affinity constant is 0.107 nM.

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Geprüfte Anwendungen

Produktinformation

83020-2-PBS bindet in IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test FDFT1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FDFT1 fusion protein Ag3743
• Vollständiger Name: farnesyl-diphosphate farnesyltransferase 1
• Berechnetes Molekulargewicht: 417 aa, 48 kDa
• GenBank-Zugangsnummer: BC029641
• Gene symbol: FDFT1
• Gene ID (NCBI): 2222
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.