SR140 Polyklonaler Antikörper

SR140 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 21399-1-AP

Synonyme

U2SURP, U2-associated protein SR140, U2 snRNP-associated SURP motif-containing protein, KIAA0332, 140 kDa Ser/Arg-rich domain protein

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  WB analysis of mouse heart using 21399-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 21399-1-AP (SR140 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21399-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21399-1-AP (SR140 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 21399-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21399-1-AP (SR140 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 21399-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21399-1-AP (SR140 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 21399-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21399-1-AP (SR140 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 21399-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21399-1-AP (SR140 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HEK-293 using 21399-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SR140 antibody (21399-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of HeLa using 21399-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SR140 antibody (21399-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of HepG2 using 21399-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SR140 antibody (21399-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of HeLa using 21399-1-AP

  Immunofluorescent analysis of Hela cells, using SR140 antibody 21399-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 21399-1-AP

  Immunofluorescent analysis of HepG2 cells, using SR140 antibody 21399-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, HeLa-Zellen, humanes Hirngewebe, L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

21399-1-AP bindet in WB, IHC, IF/ICC, ELISA SR140 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SR140 fusion protein Ag16093
• Vollständiger Name: U2-associated SR140 protein
• Berechnetes Molekulargewicht: 1029 aa, 118 kDa
• Beobachtetes Molekulargewicht: 120-140 kDa
• GenBank-Zugangsnummer: BC111692
• Gene symbol: SR140
• Gene ID (NCBI): 23350
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IHC	Immunol Res; Integrative bioinformatics and experimental analyses identify U2SURP as a novel lactylation-related prognostic signature in esophageal carcinoma; Authors - Xuan Zheng
human	WB	Sci Rep; Comprehensive systems biology analysis reveals splicing factor contributions to cutaneous melanoma progression; Authors - Shuting Zhu