SRA1 Polyklonaler Antikörper

SRA1 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 18699-1-AP

Synonyme

pp7684, SRA, SRA1, SRAP, STRAA1

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  WB analysis of A431 using 18699-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 18699-1-AP (SRA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 18699-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18699-1-AP (SRA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 18699-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed A431 cells using 18699-1-AP (SRA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

18699-1-AP bindet in WB, IHC, IF/ICC, ELISA SRA1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: steroid receptor RNA activator 1
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC156760
• Gene symbol: SRA1
• Gene ID (NCBI): 10011
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Steroid receptor RNA activator 1 (SRA1), also known as seroid receptor RNA activator protein (SRAP) or SRA, is involved in modulating the activity of multiple transcription factors including the estrogen receptor (ER) (PMID: 20398657). It may play a role in tumorigenesis (PMID: 16152589). The SRA1 gene encodes both SRA1 protein and a non-coding functional RNA that functions as part of a ribonucleoprotein complex activating steroid receptor induced transcription.

Publikationen

Species	Application	Title
mouse	WB,IHC	Atherosclerosis; Flagellar hook protein FlgE promotes macrophage activation and atherosclerosis by targeting ATP5B; Authors - Yuanyuan Li
mouse		Transl Neurodegener; Nanotechnology for microglial targeting and inhibition of neuroinflammation underlying Alzheimer's pathology; Authors - Hoda M Gebril