c-SRC Polyklonaler Antikörper

c-SRC Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

hamster, human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

65

Kat-Nr. : 11097-1-AP

Synonyme

ASV, c SRC, p60 Src, pp60c src, Proto oncogene c Src, SRC, SRC1

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Galerie der Validierungsdaten

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  WB analysis using 11097-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11097-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 11097-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of CHO using 11097-1-AP

  CHO cells were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 11097-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 11097-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11097-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 11097-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11097-1-AP (SRC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 11097-1-AP

  IP Result of anti-SRC (IP:11097-1-AP, 5ug; Detection:11097-1-AP 1:1000) with SH-SY5Y cells lysate 2900ug.

• 

  IHC staining of human breast cancer using 11097-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11097-1-AP (c-SRC Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11097-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11097-1-AP (c-SRC Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 11097-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11097-1-AP (c-SRC Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 11097-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11097-1-AP (c-SRC Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 11097-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11097-1-AP (c-SRC Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 11097-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 11097-1-AP (c-SRC Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A431 using 11097-1-AP

  Immunofluorescent analysis of A431 cells using 11097-1-AP (SRC antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of SH-SY5Y using 11097-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug c-SRC antibody (11097-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, A431-Zellen, CHO-Zellen, COLO 320-Zellen, Jurkat-Zellen, Maushirngewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Hirngewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 55 publications below
• IHC: See 8 publications below
• IF: See 1 publications below
• IP: See 4 publications below

Produktinformation

11097-1-AP bindet in WB, IP, IHC, IF, FC, ELISA c-SRC und zeigt Reaktivität mit hamster, human, Maus, Ratten

• Getestete Reaktivität: hamster, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: c-SRC fusion protein Ag1575
• Vollständiger Name: v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 55 kDa, 60 kDa
• GenBank-Zugangsnummer: BC011566
• Gene symbol: SRC
• Gene ID (NCBI): 6714
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. SRC can be detected as 60 kDa and 55 kDa (PMID: 15047935, 21461171).

Publikationen

Species	Application	Title
human	WB	Nat Genet; Pancreatic cancer risk variant in LINC00673 creates a miR-1231 binding site and interferes with PTPN11 degradation.; Authors - Jian Zheng
mouse	WB,IP	Cell Stem Cell; Amino acid catabolism regulates hematopoietic stem cell proteostasis via a GCN2-eIF2α axis.; Authors - Changzheng Li
human	WB	Sci Adv; Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes.; Authors - Patrick Han
		J Cell Biol; Metalloproteinase MT1-MMP islets act as memory devices for podosome reemergence.; Authors - Karim El Azzouzi
human		Cell Death Differ; The E3 ubiquitin ligase TRIM7 suppressed hepatocellular carcinoma progression by directly targeting Src protein.; Authors - Lihui Zhu
mouse	WB	Haematologica; NLRP3 regulates platelet integrin αIIbβ3 outside-in signaling, hemostasis and arterial thrombosis.; Authors - Jianlin Qiao

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