SRD5A1 Polyklonaler Antikörper

SRD5A1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 26001-1-AP

Synonyme

S5AR 1, SR type 1, SRD5A1, Steroid 5 alpha reductase 1

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse testis using 26001-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26001-1-AP (SRD5A1 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis of DU 145 using 26001-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 26001-1-AP (SRD5A1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 26001-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26001-1-AP (SRD5A1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 26001-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26001-1-AP (SRD5A1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 26001-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26001-1-AP (SRD5A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 26001-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26001-1-AP (SRD5A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 26001-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26001-1-AP (SRD5A1 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 26001-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26001-1-AP (SRD5A1 Antibody) at dilution of 1:100 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, DU 145-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Hodengewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

26001-1-AP bindet in WB, IHC, IF, ELISA SRD5A1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SRD5A1 fusion protein Ag23351
• Vollständiger Name: steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1)
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC007033
• Gene symbol: SRD5A1
• Gene ID (NCBI): 6715
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Steroid 5-alpha-reductase (SRD5A1) catalyzes the conversion of 17β-Hydroxyandrost-4-en-3-one into the more potent androgen, DHT. SRD5A1 is a minor component of the reductase activity in prostate although the gene was originally cloned from prostate. On the other hand, SRD5A1 appears to be the predominant isozyme of steroid 5-alpha-reductase in the scalp and elsewhere in the skin.

Publikationen

Species	Application	Title
rat	WB	Sci Rep; Pao Pereira Extract Attenuates Testosterone-Induced Benign Prostatic Hyperplasia in Rats by inhibiting 5α-Reductase.; Authors - Jiakuan Liu
mouse	WB	J Steroid Biochem Mol Biol; Syce1 and Syce3 regulate testosterone and dihydrotestosterone synthesis via steroidogenic pathways in mouse Sertoli and Leydig cells.; Authors - Qi Wang
rat	WB	J Integr Med; Rauwolfia vomitoria extract suppresses benign prostatic hyperplasia by reducing expression of androgen receptor and 5α-reductase in a rat model.; Authors - Tian Fang
mouse	IF	Biomedicines; Mixed-Culture Propagation of Uterine-Tissue-Resident Macrophages and Their Expression Properties of Steroidogenic Molecules; Authors - Kazushige Ogawa