SREBF2 Rekombinanter Antikörper

SREBF2 Rekombinant Antikörper für IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240451A12

Kat-Nr. : 83403-1-PBS

Synonyme

240451A12, bHLHd2, Class D basic helix-loop-helix protein 2, Processed sterol regulatory element-binding protein 2, SREBF 2

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  IF Staining of HeLa using 83403-1-RR (same clone as 83403-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SREBF2 antibody (83403-1-RR, Clone: 240451A12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83403-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83403-1-RR (same clone as 83403-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug SREBF2 Recombinant antibody (83403-1-RR, Clone:240451A12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83403-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83403-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83403-1-RR against Human SREBF2 were performed. The affinity constant is 0.536 nM.

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Geprüfte Anwendungen

Produktinformation

83403-1-PBS bindet in IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA SREBF2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SREBF2 fusion protein Ag28205
• Vollständiger Name: sterol regulatory element binding transcription factor 2
• Berechnetes Molekulargewicht: 124 kDa
• GenBank-Zugangsnummer: BC056158
• Gene symbol: SREBF2
• Gene ID (NCBI): 6721
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SREBF2,also named as BHLHD2, is a 1141 amino acid protein, which contains 1 bHLH domain and belongs to the SREBP family. SREBF2 localizes in the endoplasmic reticulum membrane and is ubiquitously expressed in adult and fetal tissues. SREBF2 as a transcriptional activator is required for lipid homeostasis.