SRF Monoklonaler Antikörper

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SRF Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1G8F4

Kat-Nr. : 66742-1-PBS

Synonyme

1G8F4, MCM1, Serum response factor

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  WB analysis using 66742-1-Ig (same clone as 66742-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66742-1-Ig (SRF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 66742-1-Ig (same clone as 66742-1-PBS)

  WB result of SRF antibody (66742-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRF transfected MCF-7 cells. This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66742-1-Ig (same clone as 66742-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66742-1-Ig (SRF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 66742-1-Ig (same clone as 66742-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66742-1-Ig (SRF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66742-1-Ig (same clone as 66742-1-PBS)

  WB result of SRF antibody (66742-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRF transfected HeLa cells. This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66742-1-Ig (same clone as 66742-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SRF antibody (66742-1-Ig, Clone: 1G8F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66742-1-Ig (same clone as 66742-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66742-1-Ig (SRF antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66742-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66742-1-PBS bindet in WB, IF/ICC, ELISA SRF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SRF fusion protein Ag10386
• Vollständiger Name: serum response factor (c-fos serum response element-binding transcription factor)
• Berechnetes Molekulargewicht: 508 aa, 52 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC048211
• Gene symbol: SRF
• Gene ID (NCBI): 6722
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF is required for cardiac differentiation and maturation due to the role in cardiac cell growth and muscle gene regulation. The full-length SRF protein is 67 kDa and molecular weight of cleaved fragment is 50 kDa and 20 kDa (PMID: 21769134).