SRP14 Polyklonaler Antikörper

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• KD/KO Validated

SRP14 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 11528-1-AP

Synonyme

18 kDa Alu RNA binding protein, ALURBP, SRP14

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Galerie der Validierungsdaten

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  WB analysis using 11528-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11528-1-AP (SRP14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11528-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11528-1-AP (SRP14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11528-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11528-1-AP (SRP14 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 11528-1-AP

  IP Result of anti-SRP14 (IP:11528-1-AP, 4ug; Detection:11528-1-AP 1:300) with A549 cells lysate 1600ug.

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  IF Staining of MCF-7 using 11528-1-AP

  Immunofluorescent analysis of MCF-7 cells, using SRP14 antibody 11528-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 11528-1-AP

  Immunofluorescent analysis of HepG2 cells, using SRP14 antibody 11528-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, humanes Herzgewebe
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IF: MCF-7-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IP: See 1 publications below

Produktinformation

11528-1-AP bindet in WB, IP, IF, ELISA SRP14 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SRP14 fusion protein Ag2099
• Vollständiger Name: signal recognition particle 14kDa (homologous Alu RNA binding protein)
• Berechnetes Molekulargewicht: 136 aa, 15 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC035495
• Gene symbol: SRP14
• Gene ID (NCBI): 6727
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Signal recognition particle(SRP) acts in three distinct ways: 1 it binds to the signal sequence of the nascent polypeptide to be translocated, which is exposed on the surface of the translating ribosome; 2 it temporarily retards the nascent polypeptide from further elongation; 3 it mediates docking of the SRP-ribosome-nascent polypeptide chain complex to the RER membrane via the heterodimeric SRP-receptor (SR). SRP14, also named as 18 kDa Alu RNA-binding protein, is a 136 amino acid protein. Signal recognition particle consists of a 7S RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9. SRP14 protein is a component of signal-recognition-particle that has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. SRP14 form a heterodimer with SRP9, which recognizes Alu RNA and the related 7SL RNA.

Publikationen

Species	Application	Title
human	WB	Cell; Exosome RNA Unshielding Couples Stromal Activation to Pattern Recognition Receptor Signaling in Cancer.; Authors - Barzin Y Nabet ; KD Validated
human	WB	Mol Cell; Estrogen-Related Hormones Induce Apoptosis by Stabilizing Schlafen-12 Protein Turnover.; Authors - Dianrong Li
human	WB	Genes Dev; Human intron-encoded Alu RNAs are processed and packaged into Wdr79-associated nucleoplasmic box H/ACA RNPs.; Authors - Jády Beáta E BE
human	WB,IP	Nucleic Acids Res; Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.; Authors - Evan P Booy
human	WB	Nucleic Acids Res; Implication of the SMN complex in the biogenesis and steady state level of the signal recognition particle.; Authors - Piazzon Nathalie N
human	WB	J Biol Chem; The noncoding RNA BC200 associates with polysomes to positively regulate mRNA translation in tumor cells.; Authors - Evan P Booy