SRP19 Rekombinanter Antikörper

SRP19 Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241219E9

Kat-Nr. : 84061-3-PBS

Synonyme

241219E9

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• 

  WB analysis using 84061-3-RR (same clone as 84061-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84061-3-RR (SRP19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84061-3-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 84061-3-RR (same clone as 84061-3-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SRP19 antibody (84061-3-RR, Clone: 241219E9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84061-3-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00977-1

  Cytometric bead array standard curve of MP00977-1, SRP19 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84061-1-PBS. Detection antibody: 84061-3-PBS. Standard: Ag8903. Range: 0.195-100 ng/mL.

• 

  Affinity and Kinetic Characterization of 84061-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 84061-3-PBS against Human SRP19 was performed. The affinity constant is 1.27 nM.

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Geprüfte Anwendungen

Produktinformation

84061-3-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA SRP19 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SRP19 fusion protein Ag8903
• Vollständiger Name: signal recognition particle 19kDa
• Berechnetes Molekulargewicht: 144 aa, 16 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC010947
• Gene symbol: SRP19
• Gene ID (NCBI): 6728
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The signal recognition particle (SRP) is one of the few functional small RNP particles. The SRP couples the synthesis of membrane and secretory proteins across or into the endoplasmic reticulum (ER) membrane in eukaryotes, as well as across the bacterial plasma membrane, and chloroplast thylakoid membranes. The mammalian SRP is composed of a 7S (or 7SL) RNA and six different proteins, SRP9, SRP14, SRP19, SRP54, SRP68 and SRP72. All of the components of SRP, including SRP RNA, participate directly in the overall protein targeting process. SRP19 binds directly to 7S RNA and mediates binding of the 54 kDa subunit of the SRP. SRP19 was shown to significantly enhance SRP54 attachment to helix 8 of 7SL RNA.