SRP54 Rekombinanter Antikörper

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SRP54 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241125D6

Kat-Nr. : 84014-5-PBS

Synonyme

241125D6, EC:3.6.5.4, SCN8, Signal recognition particle subunit SRP54

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  WB analysis using 84014-5-RR (same clone as 84014-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84014-5-RR (SRP54 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84014-5-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 84014-5-RR (same clone as 84014-5-PBS)

  WB result of SRP54 antibody (84014-5-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRP54 transfected MCF-7 cells. This data was developed using the same antibody clone with 84014-5-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 84014-5-RR (same clone as 84014-5-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 84014-5-RR (SRP54 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84014-5-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP00955-4

  The mean SRP54 concentration was determined to be 124.39 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load.

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  Sandwich ELISA standard curve of MP00955-4

  Sandwich ELISA standard curve of MP00955-4, Human SRP54 Recombinant Matched Antibody Pair - PBS only. 84014-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2327. 84014-5-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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  IF Staining of HeLa using 84014-5-RR (same clone as 84014-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SRP54 antibody (84014-5-RR, Clone: 241125D6 ) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84014-5-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 84014-5-RR (same clone as 84014-5-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Srp54 Recombinant Antibody (84014-5-RR, Clone:241125D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84014-5-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84014-5-RR (same clone as 84014-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Srp54 Recombinant Antibody (84014-5-RR, Clone:241125D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84014-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84014-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84014-5-RR against Human SRP54 were performed. The affinity constant is 0.287 nM.

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Geprüfte Anwendungen

Produktinformation

84014-5-PBS bindet in WB, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA SRP54 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SRP54 fusion protein Ag2327
• Vollständiger Name: signal recognition particle 54kDa
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC003389
• Gene symbol: SRP54
• Gene ID (NCBI): 6729
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM).