SRP54 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

SRP54 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

241125D6

Kat-Nr. : 84014-5-RR

Synonyme

241125D6, EC:3.6.5.4, SCN8, Signal recognition particle subunit SRP54

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  WB analysis using 84014-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84014-5-RR (SRP54 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 84014-5-RR

  WB result of SRP54 antibody (84014-5-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRP54 transfected MCF-7 cells.

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  WB analysis of human placenta using 84014-5-RR

  human placenta tissue were subjected to SDS PAGE followed by western blot with 84014-5-RR (SRP54 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84014-5-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SRP54 antibody (84014-5-RR, Clone: 241125D6 ) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HepG2 using 84014-5-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Srp54 Recombinant Antibody (84014-5-RR, Clone:241125D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HeLa using 84014-5-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Srp54 Recombinant Antibody (84014-5-RR, Clone:241125D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84014-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84014-5-RR against Human SRP54 were performed. The affinity constant is 0.287 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, humanes Plazenta-Gewebe, Jurkat-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84014-5-RR bindet in WB, IF/ICC, FC (Intra), ELISA SRP54 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SRP54 fusion protein Ag2327
• Vollständiger Name: signal recognition particle 54kDa
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC003389
• Gene symbol: SRP54
• Gene ID (NCBI): 6729
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM).