SRP54 Rekombinanter Antikörper

SRP54 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241125G1

Kat-Nr. : 84014-6-PBS

Synonyme

Signal recognition particle subunit SRP54, SCN8, EC:3.6.5.4, 241125G1

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  WB analysis using 84014-6-RR (same clone as 84014-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84014-6-RR (SRP54 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84014-6-PBS in a different storage buffer formulation.

• 

  WB analysis using 84014-6-RR (same clone as 84014-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84014-6-RR (SRP54 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84014-6-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84014-6-RR (same clone as 84014-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84014-6-RR (SRP54 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84014-6-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84014-6-RR (same clone as 84014-6-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SRP54 antibody (84014-6-RR, Clone: 241125G1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84014-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84014-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84014-6-RR against Human SRP54 were performed. The affinity constant is 4.24 nM.

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Geprüfte Anwendungen

Produktinformation

84014-6-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA SRP54 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SRP54 fusion protein Ag2327
• Vollständiger Name: signal recognition particle 54kDa
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC003389
• Gene symbol: SRP54
• Gene ID (NCBI): 6729
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM).