SRP68 Polyklonaler Antikörper

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• KD/KO Validated

SRP68 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11585-1-PBS

Synonyme

SRP 68, Signal recognition particle subunit SRP68

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  WB analysis of mouse brain using 11585-1-AP (same clone as 11585-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11585-1-AP (SRP68 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11585-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 11585-1-AP (same clone as 11585-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11585-1-AP (SRP68 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11585-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 11585-1-AP (same clone as 11585-1-PBS)

  IP result of anti-SRP68 (IP:11585-1-AP, 4ug; Detection:11585-1-AP 1:400) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 11585-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 11585-1-AP (same clone as 11585-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11585-1-AP (SRP68 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11585-1-PBS in a different storage buffer formulation.

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  IHC staining of rat testis using 11585-1-AP (same clone as 11585-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11585-1-AP (SRP68 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11585-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 11585-1-AP (same clone as 11585-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11585-1-AP (SRP68 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11585-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 11585-1-AP (same clone as 11585-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11585-1-AP (SRP68 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11585-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 11585-1-AP (same clone as 11585-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using SRP68 antibody (11585-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11585-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11585-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA SRP68 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SRP68 fusion protein Ag2177
• Vollständiger Name: signal recognition particle 68kDa
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC020238
• Gene symbol: SRP68
• Gene ID (NCBI): 6730
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of secretory proteins to the rough endoplasmic reticulum (ER) membrane. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function. SRP68 is one component of the ribonucleprotein complex, and forms a heterodimer with SRP72.