SRP9 Polyklonaler Antikörper

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• KD/KO Validated

SRP9 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 11195-1-AP

Synonyme

ALURBP, SRP9

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Galerie der Validierungsdaten

• 

  WB analysis of HepG2 using 11195-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11195-1-AP (SRP9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11195-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11195-1-AP (SRP9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11195-1-AP

  WB result of SRP9 antibody (11195-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRP9 transfected HeLa cells.

• 

  WB analysis of SKOV-3 using 11195-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11195-1-AP (SRP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 11195-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 11195-1-AP (SRP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11195-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11195-1-AP (SRP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11195-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11195-1-AP (SRP9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11195-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11195-1-AP (SRP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 11195-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 11195-1-AP (SRP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 11195-1-AP

  IP Result of anti-SRP9 (IP:11195-1-AP, 3ug; Detection:11195-1-AP 1:500) with HepG2 cells lysate 3600ug.

• 

  IHC staining of human prostate cancer using 11195-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11195-1-AP (SRP9 antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A2780-Zellen, HeLa-Zellen, HuH-7-Zellen, Maus-Kolongewebe, MCF-7-Zellen, SKOV-3-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IP: See 2 publications below

Produktinformation

11195-1-AP bindet in WB, IP, IHC, ELISA SRP9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SRP9 fusion protein Ag1683
• Vollständiger Name: signal recognition particle 9kDa
• Berechnetes Molekulargewicht: 10 kDa
• Beobachtetes Molekulargewicht: 10 kDa
• GenBank-Zugangsnummer: BC015094
• Gene symbol: SRP9
• Gene ID (NCBI): 6726
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Signal recognition particle 9(SRP9) is component of the signal recognition particle (SRP), which is a ribonucleoprotein complex that mediates the targeting of proteins to the rough endoplasmic reticulum (ER). SRP9 form a heterodimer with SRP14, which involves in arrest of the elongation of the nescent chains during targeting to ensure efficient translocation of the preprotein.

Publikationen

Species	Application	Title
human	WB	Cell; Exosome RNA Unshielding Couples Stromal Activation to Pattern Recognition Receptor Signaling in Cancer.; Authors - Barzin Y Nabet ; KD Validated
human	WB,IP	Nucleic Acids Res; Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.; Authors - Evan P Booy
	WB	Nucleic Acids Res; The pioneer round of translation ensures proper targeting of ER and mitochondrial proteins.; Authors - Joori Park
human	WB,IP	Nucleic Acids Res; Implication of the SMN complex in the biogenesis and steady state level of the signal recognition particle.; Authors - Piazzon Nathalie N
human	WB	J Proteome Res; Proteomic expression analysis of surgical human colorectal cancer tissues: up-regulation of PSB7, PRDX1, and SRP9 and hypoxic adaptation in cancer.; Authors - Rho Jung-hyun JH
human	WB	J Biol Chem; The noncoding RNA BC200 associates with polysomes to positively regulate mRNA translation in tumor cells.; Authors - Evan P Booy