SRP9 Monoklonaler Antikörper

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• KD/KO Validated

SRP9 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

1H7G10

Kat-Nr. : 66068-1-Ig

Synonyme

ALURBP, SRP9

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  WB analysis using 66068-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66068-1-Ig (SRP9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66068-1-Ig

  WB result of SRP9 antibody (66068-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRP9 transfected HeLa cells.

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  WB analysis of HeLa using 66068-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66068-1-Ig (SRP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66068-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66068-1-Ig (SRP9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of ROS1728 using 66068-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66068-1-Ig (SRP9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66068-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66068-1-Ig (SRP9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HSC-T6 cells, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, NIH/3T3-Zellen, ROS1728-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

66068-1-Ig bindet in WB, ELISA SRP9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SRP9 fusion protein Ag17153
• Vollständiger Name: signal recognition particle 9kDa
• Berechnetes Molekulargewicht: 10 kDa
• Beobachtetes Molekulargewicht: 10 kDa
• GenBank-Zugangsnummer: BC015094
• Gene symbol: SRP9
• Gene ID (NCBI): 6726
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Signal recognition particle 9(SRP9) is component of the signal recognition particle (SRP), which is a ribonucleoprotein complex that mediates the targeting of proteins to the rough endoplasmic reticulum (ER). SRP9 form a heterodimer with SRP14, which involves in arrest of the elongation of the nescent chains during targeting to ensure efficient translocation of the preprotein.

Publikationen

Species	Application	Title
human	WB	bioRxiv; Variable patterns of retrotransposition in different HeLa strains provide mechanistic insights into SINE RNA mobilization processes; Authors - John B Moldovan
human	WB	Nucleic Acids Res; Variable patterns of retrotransposition in different HeLa strains provide mechanistic insights into SINE RNA mobilization processes; Authors - John B Moldovan