SRRM1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SRRM1 Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12822-1-PBS

Synonyme

---

• 

  WB analysis of mouse liver using 12822-1-AP (same clone as 12822-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12822-1-AP (SRRM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12822-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12822-1-AP (same clone as 12822-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12822-1-AP (SRRM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12822-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 12822-1-AP (same clone as 12822-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12822-1-AP (SRRM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12822-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 12822-1-AP (same clone as 12822-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12822-1-AP (SRRM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12822-1-PBS in a different storage buffer formulation.

• 

  WB analysis of UV treated HEK-293 using 12822-1-AP (same clone as 12822-1-PBS)

  UV treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 12822-1-AP (SRRM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12822-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 12822-1-AP (same clone as 12822-1-PBS)

  IP result of anti-SRRM1 (IP:12822-1-AP, 4ug; Detection:12822-1-AP 1:300) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 12822-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

12822-1-PBS bindet in WB, IP, Indirect ELISA SRRM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SRRM1 fusion protein Ag3513
• Vollständiger Name: serine/arginine repetitive matrix 1
• Berechnetes Molekulargewicht: 904 aa, 102 kDa
• Beobachtetes Molekulargewicht: 120-160 kDa
• GenBank-Zugangsnummer: BC036187
• Gene symbol: SRRM1
• Gene ID (NCBI): 10250
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SRRM1, also named as SRM160 and POP101, contains one PWI domain and belongs to the splicing factor SR family. As part of pre- and post-splicing multiprotein mRNP complexes, SRRM1 is involved in numerous pre-mRNA processing events. It promotes constitutive and exonic splicing enhancer (ESE)-dependent splicing activation by bridging together sequence-specific (SR family proteins, SFRS4, SFRS5 and TRA2B/SFRS10) and basal snRNP (SNRP70 and SNRPA1) factors of the spliceosome. SRRM1 stimulates mRNA 3'-end cleavage independently of the formation of an exon junction complex. It binds both pre-mRNA and spliced mRNA 20-25 nt upstream of exon-exon junctions. SRRM1 binds RNA and DNA with low sequence specificity and has similar preference for either double- or single-stranded nucleic acid substrates. SRRM1, a protein of high molecular weight(120-160kd), has a heavy glycosylation level and is normally highly phosphorylated. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SRRM1.