SRRT Polyklonaler Antikörper

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• KD/KO Validated

SRRT Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20353-1-PBS

Synonyme

serrate, ASR2, Arsenite-resistance protein 2, Arsenite resistance protein 2, ARS2

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  WB analysis using 20353-1-AP (same clone as 20353-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20353-1-AP (SRRT antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20353-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 20353-1-AP (same clone as 20353-1-PBS)

  WB result of SRRT antibody (20353-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRRT transfected NIH/3T3 cells. This data was developed using the same antibody clone with 20353-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 20353-1-AP (same clone as 20353-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20353-1-AP (SRRT antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20353-1-PBS in a different storage buffer formulation.

• 

  IP experiment of Jurkat using 20353-1-AP (same clone as 20353-1-PBS)

  IP result of anti-SRRT (IP:20353-1-AP, 4ug; Detection:20353-1-AP 1:30000) with Jurkat cells lysate 1440 ug. This data was developed using the same antibody clone with 20353-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 20353-1-AP (same clone as 20353-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SRRT antibody (20353-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 20353-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20353-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA SRRT und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SRRT fusion protein Ag14196
• Vollständiger Name: serrate RNA effector molecule homolog (Arabidopsis)
• Berechnetes Molekulargewicht: 876 aa, 101 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC000082
• Gene symbol: SRRT
• Gene ID (NCBI): 51593
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SRRT (Serrate RNA effector molecule homolog) is also named as ARS2 (Arsenite-resistance protein 2). ARS2/SRRT is an essential eukaryotic protein that has emerged as a critical factor in the sorting of functional from non-functional RNA polymerase II (Pol II) transcripts (PMID: 34060620). SRRT protein is a key component of the nuclear cap-binding complex (CBC), which allows it to modulate the expression of various miRNAs. The SRRT protein is widely expressed throughout the eukaryotes, but not in budding yeast. SRRT has been shown to play a vital role in stem-cell renewal, proliferation, hematopoiesis, and RNA-mediated gene silencing and regulation of miRNA biogenesis (PMID: 37345203). SRRT is involved in cellular proliferation and colony formation (tumorigenicity). Similarly, high SRRT expression has been found in cholangiocarcinoma. ARS2 showed ubiquitous expression, with the highest levels in mouse testes, heart, and liver and human heart and skeletal muscle (PMID: 18086880).