SRY Monoklonaler Antikörper

SRY Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2E9A4

Kat-Nr. : 67349-1-PBS

Synonyme

2E9A4, sex determining region Y, Sex-determining region Y protein, Testis-determining factor

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  WB analysis of LNCaP using 67349-1-Ig (same clone as 67349-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67349-1-Ig (SRY antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67349-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67349-1-Ig (same clone as 67349-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67349-1-Ig (SRY antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. SRY negative cell line HeLa cell line was served as negative control. This data was developed using the same antibody clone with 67349-1-PBS in a different storage buffer formulation.

• 

  WB analysis of DU 145 using 67349-1-Ig (same clone as 67349-1-PBS)

  DU 145 cells were subjected to SDS PAGE followed by western blot with 67349-1-Ig (SRY antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67349-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67349-1-PBS bindet in WB, Indirect ELISA SRY und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SRY fusion protein Ag12610
• Vollständiger Name: sex determining region Y
• Berechnetes Molekulargewicht: 204 aa, 24 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC074924
• Gene symbol: SRY
• Gene ID (NCBI): 6736
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SRY (sex-determining region Y protein) is a tran-scriptional activator required for male sex determination in mammals. This protein, also referred to as testis-determining factor (TDF), is an HMG box protein that initiates the formation of testis from undifferentiated gonad. The DNA-binding activity of SRY is required for normal testis formation. This DNA-binding activity is thought to be regulated by PKA, which phosphorylates SRY in vivo. Mutations in SRY have been associated with 46,XY gonadal dysgenesis, in which the gonads fail to develop in XY phenotypic females.