SSBP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SSBP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(29)

Kat-Nr. : 12212-1-AP

Synonyme

Mt SSB, MtSSB, Mt-SSB, PWP1 interacting protein 17, PWP1-interacting protein 17

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  WB analysis of A549 using 12212-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12212-1-AP (SSBP1 antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of A549 using 12212-1-AP

  WB result of SSBP1 antibody (12212-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SSBP1 transfected A549 cells.

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  WB analysis using 12212-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12212-1-AP (SSBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat pancreas using 12212-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 12212-1-AP (SSBP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 12212-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12212-1-AP (SSBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 12212-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12212-1-AP (SSBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A375 using 12212-1-AP

  IP result of anti-SSBP1 (IP:12212-1-AP, 4ug; Detection:12212-1-AP 1:4000) with A375 cells lysate 1440 ug.

• 

  IHC staining of human stomach cancer using 12212-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12212-1-AP (SSBP1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 12212-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12212-1-AP (SSBP1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A375 using 12212-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using 12212-1-AP (SSBP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, A375-Zellen, Caco-2-Zellen, Maus-Pankreasgewebe, Ratten-Pankreasgewebe
• Erfolgreiche IP: A375-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A375-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 25 publications below
• IHC: See 3 publications below
• IF: See 4 publications below
• ChIP: See 1 publications below

Produktinformation

12212-1-AP bindet in WB, IHC, IF/ICC, IP, ChIP, ELISA SSBP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SSBP1 fusion protein Ag2860
• Vollständiger Name: single-stranded DNA binding protein 1
• Berechnetes Molekulargewicht: 148 aa, 17 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC000895
• Gene symbol: SSBP1
• Gene ID (NCBI): 6742
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SSBP1 is a housekeeping gene involved in mitochondrial biogenesis. it binds preferentially and cooperatively to ss-DNA in the maintainance of genome stability. SSBP1 acted as a homotetramer to stabilize the displaced single strand of the normal and expanded displacement loop (D loop) during mtDNA replication, thus preventing formation of secondary single-stranded DNA structures, which could stop the gamma-DNA polymerase

Publikationen

Species	Application	Title
	WB	Nature; Stress response silencing by an E3 ligase mutated in neurodegeneration; Authors - Diane L Haakonsen
human	WB	Cell; MOF Acetyl Transferase Regulates Transcription and Respiration in Mitochondria.; Authors - Aindrila Chatterjee
human	WB	Nat Cell Biol; Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics.; Authors - Timo Rey
human	WB	Mol Cell; Single-Molecule Analysis of mtDNA Replication Uncovers the Basis of the Common Deletion.; Authors - Aaron F Phillips
		Sci Adv; The mitochondrial single-stranded DNA binding protein is essential for initiation of mtDNA replication.; Authors - Min Jiang ; KO Validated
human	IHC	Nucleic Acids Res; Top3α is the replicative topoisomerase in mitochondrial DNA replication.; Authors - Anu Hangas