SSBP1 Rekombinanter Antikörper

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• KD/KO Validated

SSBP1 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242519C5

Kat-Nr. : 84939-3-PBS

Synonyme

242519C5, Mt SSB, MtSSB, Mt-SSB, PWP1 interacting protein 17

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  WB analysis using 84939-3-RR (same clone as 84939-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84939-3-RR (SSBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84939-3-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 84939-3-RR (same clone as 84939-3-PBS)

  WB result of SSBP1 antibody (84939-3-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SSBP1 transfected A549 cells. This data was developed using the same antibody clone with 84939-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 84939-3-RR (same clone as 84939-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84939-3-RR (SSBP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84939-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 84939-3-RR (same clone as 84939-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84939-3-RR (SSBP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84939-3-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84939-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84939-3-RR against Human SSBP1 were performed. The affinity constant is 0.144 nM.

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Geprüfte Anwendungen

Produktinformation

84939-3-PBS bindet in WB, IHC, Indirect ELISA SSBP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SSBP1 fusion protein Ag2860
• Vollständiger Name: single-stranded DNA binding protein 1
• Berechnetes Molekulargewicht: 148 aa, 17 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC000895
• Gene symbol: SSBP1
• Gene ID (NCBI): 6742
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SSBP1 is a housekeeping gene involved in mitochondrial biogenesis. it binds preferentially and cooperatively to ss-DNA in the maintainance of genome stability. SSBP1 acted as a homotetramer to stabilize the displaced single strand of the normal and expanded displacement loop (D loop) during mtDNA replication, thus preventing formation of secondary single-stranded DNA structures, which could stop the gamma-DNA polymerase.