SSBP1 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

SSBP1 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242519C5

Kat-Nr. : 84939-3-RR

Synonyme

242519C5, Mt SSB, MtSSB, Mt-SSB, PWP1 interacting protein 17

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  WB analysis of A549 using 84939-3-RR

  WB result of SSBP1 antibody (84939-3-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SSBP1 transfected A549 cells.

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  WB analysis using 84939-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84939-3-RR (SSBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 84939-3-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84939-3-RR (SSBP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 84939-3-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84939-3-RR (SSBP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84939-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84939-3-RR against Human SSBP1 were performed. The affinity constant is 0.144 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, A549-Zellen, Caco-2-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84939-3-RR bindet in WB, IHC, ELISA SSBP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SSBP1 fusion protein Ag2860
• Vollständiger Name: single-stranded DNA binding protein 1
• Berechnetes Molekulargewicht: 148 aa, 17 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC000895
• Gene symbol: SSBP1
• Gene ID (NCBI): 6742
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SSBP1 is a housekeeping gene involved in mitochondrial biogenesis. it binds preferentially and cooperatively to ss-DNA in the maintainance of genome stability. SSBP1 acted as a homotetramer to stabilize the displaced single strand of the normal and expanded displacement loop (D loop) during mtDNA replication, thus preventing formation of secondary single-stranded DNA structures, which could stop the gamma-DNA polymerase.