TRAPA/SSR1 Polyklonaler Antikörper

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• KD/KO Validated

TRAPA/SSR1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Rezensionen (1)

Kat-Nr. : 10583-1-AP

Synonyme

SSR1, TRAP alpha, PSEC0262, SSR alpha, SSR-alpha

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  WB analysis using 10583-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10583-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10583-1-AP

  WB result of TRAPA/SSR1 antibody (10583-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAPA/SSR1 transfected HepG2 cells.

• 

  WB analysis of human liver using 10583-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:250 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10583-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10583-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 10583-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 10583-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 10583-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 10583-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 10583-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TRAPA/SSR1 antibody (10583-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COS-7-Zellen, HepG2-Zellen, humanes Lebergewebe, L02-Zellen, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

10583-1-AP bindet in WB, IHC, IF/ICC, ELISA TRAPA/SSR1 und zeigt Reaktivität mit Affe, human, Maus

• Getestete Reaktivität: Affe, human, Maus
• In Publikationen genannte Reaktivität: human, Affe, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRAPA/SSR1 fusion protein Ag0878
• Vollständiger Name: signal sequence receptor, alpha
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC007710
• Gene symbol: TRAPA
• Gene ID (NCBI): 6745
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRAPA (translocon-associated protein subunit alpha), also known as SSR1 (signal sequence receptor subunit alpha), is a 34-kDa single-spanning membrane glycoprotein of the endoplasmic reticulum (ER) (PMID: 8050590). It is a part of the tetrameric TRAP complex (TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma) which functions in regulating the retention of ER resident proteins (PMID: 7916687). The TRAP complex is also involved in endoplasmic reticulum-associated degradation (ERAD) (PMID: 17380188).

Publikationen

Species	Application	Title
human	IF	Nature; ER proteins decipher the tubulin code to regulate organelle distribution; Authors - Pengli Zheng
human	WB	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
human	WB	Sci Adv; The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress.; Authors - Chatchai Phoomak
human	IF	Biosens Bioelectron; Live imaging of RNA and RNA splicing in mammalian cells via the dcas13a-SunTag-BiFC system.; Authors - Mao Chen
human	WB	FASEB J; GPR37 is processed in the N-terminal ectodomain by ADAM10 and furin.; Authors - S Orvokki Mattila
rat	WB,IF	Exp Cell Res; Microdomains of endoplasmic reticulum within the sarcoplasmic reticulum of skeletal myofibers.; Authors - Kaakinen Mika M

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chatchai (Verified Customer) (09-26-2019)

This anti-SSR1 is very good, presents the specific band at the right size of the protein.

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
• Primary Antibody Dilution: 1:500 for WB
• Cell Tissue Type: A549, HEK-293