SSR4 Polyklonaler Antikörper

SSR4 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 11655-2-AP

Synonyme

TRAP-delta, TRAPD, Translocon-associated protein subunit delta, SSR-delta

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  WB analysis using 11655-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11655-2-AP (SSR4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 11655-2-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11655-2-AP (SSR4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11655-2-AP

  IP result of anti-SSR4 (IP:11655-2-AP, 4ug; Detection:11655-2-AP 1:300) with HeLa cells lysate 2600 ug.

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  IHC staining of human pancreas cancer using 11655-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11655-2-AP (SSR4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 11655-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11655-2-AP (SSR4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11655-2-AP

  Immunofluorescent analysis of HeLa cells using 11655-2-AP (SSR4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, humanes Plazenta-Gewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 3 publications below

Produktinformation

11655-2-AP bindet in WB, IHC, IF/ICC, IP, ELISA SSR4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SSR4 fusion protein Ag2250
• Vollständiger Name: signal sequence receptor, delta (translocon-associated protein delta)
• Berechnetes Molekulargewicht: 173 aa, 19 kDa
• Beobachtetes Molekulargewicht: 19 kDa
• GenBank-Zugangsnummer: BC003371
• Gene symbol: SSR4
• Gene ID (NCBI): 6748
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Signal sequence receptor subunit delta (SSR4, or TRAPD) gene encodes the translocon-associated proteins subunit delta, which is about 19-kDa and is located at endoplasmic reticulum (ER) membrane, the translocon-associated proteins complex functions to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. TRAP-delta can form heterotetramer with other TRAP homolog TRAP-alpha, TRAP-beta and TRAP-gamma.

Publikationen

Species	Application	Title
human	WB	Sci Adv; The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress.; Authors - Chatchai Phoomak
human	IHC	Expert Rev Mol Diagn; SSR4 as a prognostic biomarker and related with immune infiltration cells in colon adenocarcinoma.; Authors - Weiwei He
human	IHC	Cancer Med; Predicting Outcomes in Esophageal Squamous Cell Carcinoma Using scRNA-Seq and Bulk RNA-Seq: A Model Development and Validation Study; Authors - Jiaqi Zhang
human	WB	J Cancer Prev; Identification of Translocon-associated Protein Delta as An Oncogene in Human Colorectal Cancer Cells; Authors - Darshika Amarakoon
human	IHC	Front Immunol; Unveiling SSR4: a promising biomarker in esophageal squamous cell carcinoma; Authors - Jiaqi Zhang
human	WB	Eur J Pharmacol; Eeyarestatin I (ESI)-induced ERAD inhibition exhibits anti-cancer activity through multiple mechanisms in human colorectal cancer cells; Authors - Jing Peng