SSRP1 Monoklonaler Antikörper

SSRP1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

3D3H4

Kat-Nr. : 67313-1-Ig

Synonyme

FACT, FACT 80 kDa subunit, FACT complex subunit SSRP1, FACT80, FACTp80, hSSRP1, SSRP1, T160

---

Galerie der Validierungsdaten

• 

  WB analysis using 67313-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67313-1-Ig (SSRP1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 67313-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67313-1-Ig (SSRP1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 4T1 using 67313-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67313-1-Ig (SSRP1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 67313-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67313-1-Ig (SSRP1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67313-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67313-1-Ig (SSRP1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67313-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67313-1-Ig (SSRP1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 67313-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67313-1-Ig (SSRP1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 67313-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67313-1-Ig (SSRP1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunhistochemie (IHC): IHC : 1:5000-1:20000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

67313-1-Ig bindet in WB, IHC, IF, ELISA SSRP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SSRP1 fusion protein Ag8318
• Vollständiger Name: structure specific recognition protein 1
• Berechnetes Molekulargewicht: 81 kDa
• Beobachtetes Molekulargewicht: 86 kDa
• GenBank-Zugangsnummer: BC005116
• Gene symbol: SSRP1
• Gene ID (NCBI): 6749
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IHC	Biomed Pharmacother; CBL0137 activates ROS/BAX signaling to promote caspase-3/GSDME-dependent pyroptosis in ovarian cancer cells; Authors - Chang Yang ; KD Validated