SSRP1 Rekombinanter Antikörper

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• KD/KO Validated

SSRP1 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

242102H8

Kat-Nr. : 84673-5-RR

Synonyme

242102H8, Chromatin-specific transcription elongation factor 80 kDa subunit, Facilitates chromatin transcription complex 80 kDa subunit, Facilitates chromatin transcription complex subunit SSRP1, FACT

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  WB analysis of HEK-293 using 84673-5-RR

  WB result of SSRP1 antibody (84673-5-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SSRP1 transfected HEK-293 cells.

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  WB analysis using 84673-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84673-5-RR (SSRP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 84673-5-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug SSRP1 Recombinant antibody (84673-5-RR, Clone:242102H8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of SH-SY5Y using 84673-5-RR

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug SSRP1 Recombinant antibody (84673-5-RR, Clone:242102H8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84673-5-RR bindet in WB, FC (Intra), ELISA SSRP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SSRP1 fusion protein Ag8318
• Vollständiger Name: structure specific recognition protein 1
• Berechnetes Molekulargewicht: 81 kDa
• Beobachtetes Molekulargewicht: 86 kDa
• GenBank-Zugangsnummer: BC005116
• Gene symbol: SSRP1
• Gene ID (NCBI): 6749
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.