SSRP1 Monoklonaler Antikörper

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• KD/KO Validated

SSRP1 Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3D3H4

Kat-Nr. : CL488-67313

Synonyme

FACT 80 kDa subunit, FACT, Facilitates chromatin transcription complex subunit SSRP1, Facilitates chromatin transcription complex 80 kDa subunit, Chromatin-specific transcription elongation factor 80 kDa subunit

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  IF Staining of HepG2 using CL488-67313

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 SSRP1 antibody (CL488-67313, Clone: 3D3H4 ) at dilution of 1:100.

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  IF Staining of HepG2 using CL488-67313

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 SSRP1 antibody (CL488-67313, Clone: 3D3H4 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-67313

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SSRP1 (CL488-67313, Clone:3D3H4) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-67313 bindet in IF/ICC, FC (Intra) SSRP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SSRP1 fusion protein Ag8318
• Vollständiger Name: structure specific recognition protein 1
• Berechnetes Molekulargewicht: 81 kDa
• GenBank-Zugangsnummer: BC005116
• Gene symbol: SSRP1
• Gene ID (NCBI): 6749
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.