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SSSCA1 Monoklonaler Antikörper

SSSCA1 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D2E8

Kat-Nr. : 68423-1-PBS

Synonyme

Autoantigen p27, p27, SSSCA1, ZNRD2

Filter:
  • Western Blot (WB) analysis of various lysates using SSSCA1 Monoclonal antibody (68423-1-Ig)

    WB analysis using 68423-1-Ig (same clone as 68423-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68423-1-Ig (SSSCA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68423-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SSSCA1 Monoclonal antibody (68423-1-Ig)

    IF Staining of HeLa using 68423-1-Ig (same clone as 68423-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SSSCA1 antibody (68423-1-Ig, Clone: 1D2E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68423-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

68423-1-PBS bindet in WB, IF/ICC, Indirect ELISA SSSCA1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen SSSCA1 fusion protein Ag16783
Vollständiger Name Sjogren syndrome/scleroderma autoantigen 1
Berechnetes Molekulargewicht 199 aa, 21 kDa
Beobachtetes Molekulargewicht23 kDa
GenBank-ZugangsnummerBC014791
Gene symbol SSSCA1
Gene ID (NCBI) 10534
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SSSCA1, also named as Autoantigen p27, is centromere-associated protein. It may induce anti-centromere antibodies. SSSCA1 plays a role in mitosis. It's involved in Sjogren syndrome/scleroderma. The calculated MW of SSSCA1 is 21.5 kDa (native form), with modification the MW is about 23-27 kDa.