SSTR5 Monoklonaler Antikörper

SSTR5 Monoklonal Antikörper für WB, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF-P, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

CloneNo.

2B10D8

Kat-Nr. : 66772-1-Ig

Synonyme

somatostatin receptor 5, Somatostatin receptor type 5, SS 5 R, SS5 R, SS5R, SST5, SSTR5

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  WB analysis using 66772-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66772-1-Ig (SSTR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66772-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66772-1-Ig (SSTR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse kidney using 66772-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using SSTR5 antibody (66772-1-Ig, Clone: 2B10D8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse kidney using 66772-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using SSTR5 antibody (66772-1-Ig, Clone: 2B10D8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HSC-T6 cells, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Neuro-2a-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF-P: Mausnierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

66772-1-Ig bindet in WB, IF-P, CoIP, ELISA SSTR5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SSTR5 fusion protein Ag18615
• Vollständiger Name: somatostatin receptor 5
• Berechnetes Molekulargewicht: 364 aa, 39 kDa
• Beobachtetes Molekulargewicht: 41 kDa
• GenBank-Zugangsnummer: BC146576
• Gene symbol: SSTR5
• Gene ID (NCBI): 6755
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Somatostatin is a widely distributed peptide with a broad range of biological actions. Two biological forms of somatostatin exist: somatostatin-14 and -28. Somatostatin receptors (SSTR1, 2A and B, 3, 4 and 5) belong to the G protein coupled receptor family and have a wide expression pattern in both normal tissues and solid tumors (PMID: 23872332). SSTR5 has greater affinity for somatostatin-28 than somatostatin-14 (PMID: 8373420).

Publikationen

Species	Application	Title
		EMBO J; Somatostatin triggers local cAMP and Ca2+ signaling in primary cilia to modulate pancreatic β-cell function; Authors - Ceren Incedal Nilsson
rat	WB	Phytomedicine; Differential effect of polysaccharide and nonpolysaccharide components in Sijunzi decoction on spleen deficiency syndrome and their mechanisms.; Authors - Ping Ma
mouse	WB	Endocr J; Differential effects of β-arrestin1 and β-arrestin2 on somatostatin receptors in murine AtT-20 corticotroph tumor cells.; Authors - Kazunori Kageyama
human	IF	Front Endocrinol (Lausanne); Dimerization of GPCRs: Novel insight into the role of FLNA and SSAs regulating SST2 and SST5 homo- and hetero-dimer formation; Authors - Donatella Treppiedi
human	WB,CoIP	Cell Death Discov; Repositioning Lomitapide to block ZDHHC5-dependant palmitoylation on SSTR5 leads to anti-proliferation effect in preclinical pancreatic cancer models; Authors - Yumeng Wang