SSU72 Polyklonaler Antikörper

SSU72 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 15434-1-AP

Synonyme

RNA polymerase II subunit A C-terminal domain phosphatase SSU72, PNAS-120, PNAS 120, HSPC182, EC:3.1.3.16

---

• 

  WB analysis using 15434-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 15434-1-AP (SSU72 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 15434-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 15434-1-AP (SSU72 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15434-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15434-1-AP (SSU72 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 15434-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15434-1-AP (SSU72 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 15434-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15434-1-AP (SSU72 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 15434-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15434-1-AP (SSU72 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 15434-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15434-1-AP (SSU72 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 15434-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15434-1-AP (SSU72 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15434-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15434-1-AP (SSU72 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15434-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15434-1-AP (SSU72 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15434-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15434-1-AP (SSU72 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Mammakarzinomgewebe, humanes Nierengewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

15434-1-AP bindet in WB, IHC, ELISA SSU72 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SSU72 fusion protein Ag7699
• Vollständiger Name: SSU72 RNA polymerase II CTD phosphatase homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC008070
• Gene symbol: SSU72
• Gene ID (NCBI): 29101
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Sci Adv; EIF3D safeguards the homeostasis of key signaling pathways in human primed pluripotency; Authors - Chikako Okubo
		Mol Cell; Mediator regulates transcriptional termination through crosstalk with pre-mRNA 3' end processing factors; Authors - Chengjiang Lu