IL-1RL1/ST2 Polyklonaler Antikörper

IL-1RL1/ST2 Polyklonal Antikörper für WB, FC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30194-1-AP

Synonyme

IL1RL1, DER4, EC:3.2.2.6, FIT 1, IL-1RL1

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  WB analysis using 30194-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30194-1-AP (IL-1RL1/ST2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  FC experiment of HEK-293 using 30194-1-AP

  1x10^6 HEK-293 cells were surface stained with 0.4 ug IL-1RL1/ST2 Polyclonal antibody (30194-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) (red), or 0.4 ug rabbit IgG isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in FC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30194-1-AP bindet in WB, FC, ELISA IL-1RL1/ST2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL-1RL1/ST2 fusion protein Eg0116
• Vollständiger Name: interleukin 1 receptor-like 1
• Berechnetes Molekulargewicht: 63 kDa, 37 kDa, 30 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC030975
• Gene symbol: IL1RL1
• Gene ID (NCBI): 9173
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ST2, also known as IL1RL1, is a member of the IL-1 superfamily and serves as the receptor for IL-33. It plays a major role in immune and inflammatory responses. ST2 is expressed by various types of immune cells including Th2 cells, mast cells, type 2 innate lymphoid cells, eosinophils, basophils, NK cells, and non-immune cells, including cardiomyocytes. Two forms of ST2 exist: a membrane-bound form and a soluble form (sST2). The membrane-bound form activates the MyD88/NF-κB signaling pathway to enhance mast cell, Th2, regulatory T cell (Treg), and innate lymphoid cell type 2 functions. sST2 acts as a decoy receptor. (PMID: 27055914; 23999434; 28484466)