ST3GAL2 Polyklonaler Antikörper

ST3GAL2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13620-1-AP

Synonyme

Alpha 2,3 ST 2, Gal NAc6S, Sialyltransferase 4B, SIAT4 B, SIAT4B, ST3Gal II, ST3GAL2, ST3GalA.2, ST3GALII

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Galerie der Validierungsdaten

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  WB analysis of human colon using 13620-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 13620-1-AP (ST3GAL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13620-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13620-1-AP (ST3GAL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13620-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13620-1-AP (ST3GAL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 13620-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 13620-1-AP (ST3GAL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13620-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13620-1-AP (ST3GAL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 13620-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13620-1-AP (ST3GAL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat heart using 13620-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 13620-1-AP (ST3GAL2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 13620-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 13620-1-AP (ST3GAL2 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 13620-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 13620-1-AP (ST3GAL2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 13620-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 13620-1-AP (ST3GAL2 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Kolongewebe, HL-60-Zellen, humanes Herzgewebe, humanes Lebergewebe, humanes Milzgewebe, K-562-Zellen
• Erfolgreiche Detektion in IHC: Rattenherzgewebe, humanes Hautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

13620-1-AP bindet in WB, IHC, ELISA ST3GAL2 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ST3GAL2 fusion protein Ag4528
• Vollständiger Name: ST3 beta-galactoside alpha-2,3-sialyltransferase 2
• Berechnetes Molekulargewicht: 350 aa, 40 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC036777
• Gene symbol: ST3GAL2
• Gene ID (NCBI): 6483
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Beta-galactoside alpha-2,3-sialyltransferase 2 (ST3GAL2) is a sialyltransferase (ST) that transfers sialic acid from the activated donor CMP-sialic acid to the glycan terminus of a glycolipid or glycoprotein. ST3GAL2 contains two putative N-glycosylation sites (Asn92 and Asn211), which are mainly responsible for GD1a and GT1b ganglioside biosynthesis in the brain. ST3GAL2 belongs to the sialyltransferase family, which mediates the terminal modification of glycoproteins and glycolipids. ST3GAL2 has been found to play a role in obesity, aging, and malignant diseases (PMID: 34319453). The calculated molecular weight of ST3GAL2 is 40 kDa, but the observed is 55 kDa, which may be the glycosylation form of ST3GAL2.