ST3GAL2 Polyklonaler Antikörper

ST3GAL2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 13620-1-AP

Synonyme

Alpha 2,3-ST 2, Beta-galactoside alpha-2,3-sialyltransferase 2, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2, EC:2.4.3.2, EC:2.4.3.4

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  WB analysis using 13620-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13620-1-AP (ST3GAL2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of rat heart using 13620-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 13620-1-AP (ST3GAL2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin using 13620-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 13620-1-AP (ST3GAL2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 13620-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 13620-1-AP (ST3GAL2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of rat heart using 13620-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 13620-1-AP (ST3GAL2 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: Rattenherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

13620-1-AP bindet in WB, IHC, ELISA ST3GAL2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ST3GAL2 fusion protein Ag4528
• Vollständiger Name: ST3 beta-galactoside alpha-2,3-sialyltransferase 2
• Berechnetes Molekulargewicht: 350 aa, 40 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC036777
• Gene symbol: ST3GAL2
• Gene ID (NCBI): 6483
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Beta-galactoside alpha-2,3-sialyltransferase 2 (ST3GAL2) is a sialyltransferase (ST) that transfers sialic acid from the activated donor CMP-sialic acid to the glycan terminus of a glycolipid or glycoprotein. ST3GAL2 contains two putative N-glycosylation sites (Asn92 and Asn211), which are mainly responsible for GD1a and GT1b ganglioside biosynthesis in the brain. ST3GAL2 belongs to the sialyltransferase family, which mediates the terminal modification of glycoproteins and glycolipids. ST3GAL2 has been found to play a role in obesity, aging, and malignant diseases (PMID: 34319453). The calculated molecular weight of ST3GAL2 is 40 kDa.

Publikationen

Species	Application	Title
mouse,human	WB	Int J Biol Macromol; Regulation of autophagy by ST3GAL2-mediated α2-3 sialylated glycosphingolipids in hepatic encephalopathy; Authors - Xiaocheng Li