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ST3GAL5 Polyklonaler Antikörper
ST3GAL5 Polyklonal Antikörper für WB, IHC, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus
Anwendung
WB, IHC, IF, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 14614-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HEK-293-Zellen, HeLa-Zellen, humanes Plazenta-Gewebe, humanes Hodengewebe, Jurkat-Zellen |
Erfolgreiche Detektion in IHC | Maus-Skelettmuskelgewebe, humanes Skelettmuskelgewebe, humanes Hautgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunhistochemie (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 1 publications below |
WB | See 5 publications below |
IHC | See 4 publications below |
IF | See 2 publications below |
Produktinformation
14614-1-AP bindet in WB, IHC, IF, ELISA ST3GAL5 und zeigt Reaktivität mit human, Maus
Getestete Reaktivität | human, Maus |
In Publikationen genannte Reaktivität | human, Maus |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | ST3GAL5 fusion protein Ag6207 |
Vollständiger Name | ST3 beta-galactoside alpha-2,3-sialyltransferase 5 |
Berechnetes Molekulargewicht | 48 kDa |
Beobachtetes Molekulargewicht | 45-50 kDa |
GenBank-Zugangsnummer | BC065936 |
Gene symbol | ST3GAL5 |
Gene ID (NCBI) | 8869 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
ST3GAL5 is also named as SIAT9, GM3 synthase, ST3Gal V, Sialyltransferase 9 and belongs to the glycosyltransferase 29 family. ST3GAL5 transfers the sialyl group (N-acetyl-alpha-neuraminyl or NeuAc) from CMP-NeuAc to the non-reducing terminal galactose of glycosphingolipids forming gangliosides (PMID:9822625, PMID:16934889). SIAT9 is mainly involved in the biosynthesis of ganglioside GM3 but can also use different glycolipids as substrate acceptors such as D-galactosylceramide (GalCer), asialo-GM2 (GA2) and asialo-GM1 (GA1) (PMID:16934889). ST3GAL5 is highly expressed in brain, skeletal muscle, placenta, and testis. And mRNA of ST3GAL5 is widely distributed in human brain, but slightly elevated expression was observed in the cerebral cortex, temporal lobe, and putamen (PMID:9822625). ST3GAL5 has 3 isoforms with the molecular mass of 45, 46, and 48 kDa. Sometimes the band of 70 kDa can also be observed, which may be a glycosylated form of ST3GAL5 (PMID: 34943806, PMID: 26458842).
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for ST3GAL5 antibody 14614-1-AP | Protokoll herunterladen |
IHC protocol for ST3GAL5 antibody 14614-1-AP | Protokoll herunterladenl |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Front Immunol Identification of ST3GAL5 as a prognostic biomarker correlating with CD8+ T cell exhaustion in clear cell renal cell carcinoma | ||
Front Cell Dev Biol PRC2-dependent regulation of ganglioside expression during dedifferentiation contributes to the proliferation and migration of vascular smooth muscle cells | ||
iScience C-Myc-induced hypersialylation of small cell lung cancer facilitates pro-tumoral phenotypes of macrophages | ||
Mol Oncol Exosomes secreted by ST3GAL5high cancer cells promote peritoneal dissemination by establishing a pre-metastatic microenvironment
| ||
Int Immunopharmacol Identification and validation of sialyltransferase ST3Gal5 in bladder cancer through bioinformatics and experimental analysis | ||
Cancer Cell Int Comprehensive analysis of α2,3-sialyltransferases as prognostic biomarkers and immunotherapy targets in kidney renal clear cell carcinoma |