ST3GAL5 Polyklonaler Antikörper

ST3GAL5 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 14614-1-AP

Synonyme

Ganglioside GM3 synthase, Sialyltransferase 9, SIAT9, SIATGM3S, ST3Gal V, ST3GAL5, ST3GalV

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 14614-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14614-1-AP (ST3GAL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 14614-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14614-1-AP (ST3GAL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 14614-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14614-1-AP (ST3GAL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14614-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14614-1-AP (ST3GAL5 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14614-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14614-1-AP (ST3GAL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skeletal muscle using 14614-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14614-1-AP (ST3GAL5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 14614-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14614-1-AP (ST3GAL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 14614-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14614-1-AP (ST3GAL5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 14614-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14614-1-AP (ST3GAL5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 14614-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 14614-1-AP (ST3GAL5 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 14614-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 14614-1-AP (ST3GAL5 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, humanes Hodengewebe, humanes Plazenta-Gewebe, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe, humanes Hautgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

14614-1-AP bindet in WB, IHC, IF, ELISA ST3GAL5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ST3GAL5 fusion protein Ag6207
• Vollständiger Name: ST3 beta-galactoside alpha-2,3-sialyltransferase 5
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC065936
• Gene symbol: ST3GAL5
• Gene ID (NCBI): 8869
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ST3GAL5 is also named as SIAT9, GM3 synthase, ST3Gal V, Sialyltransferase 9 and belongs to the glycosyltransferase 29 family. ST3GAL5 transfers the sialyl group (N-acetyl-alpha-neuraminyl or NeuAc) from CMP-NeuAc to the non-reducing terminal galactose of glycosphingolipids forming gangliosides (PMID:9822625, PMID:16934889). SIAT9 is mainly involved in the biosynthesis of ganglioside GM3 but can also use different glycolipids as substrate acceptors such as D-galactosylceramide (GalCer), asialo-GM2 (GA2) and asialo-GM1 (GA1) (PMID:16934889). ST3GAL5 is high expression in brain, skeletal muscle, placenta, and testis. And mRNA of ST3GAL5 is widely distributed in human brain, but slightly elevated expression was observed in the cerebral cortex, temporal lobe, and putamen (PMID:9822625). ST3GAL5 has 3 isoforms with the molecular mass of 45, 46, and 48 kDa. Sometimes the band of 70 kDa can also be observed, which may be a glycosylated form of ST3GAL5 (PMID: 34943806, PMID: 26458842).

Publikationen

Species	Application	Title
human	IHC	Front Immunol; Identification of ST3GAL5 as a prognostic biomarker correlating with CD8+ T cell exhaustion in clear cell renal cell carcinoma; Authors - Jiakuan Liu
human	WB	Front Cell Dev Biol; PRC2-dependent regulation of ganglioside expression during dedifferentiation contributes to the proliferation and migration of vascular smooth muscle cells; Authors - Norihiko Sasaki
human	WB	iScience; C-Myc-induced hypersialylation of small cell lung cancer facilitates pro-tumoral phenotypes of macrophages; Authors - Lin Tian
human,mouse	WB,IHC,IF	Mol Oncol; Exosomes secreted by ST3GAL5high cancer cells promote peritoneal dissemination by establishing a pre-metastatic microenvironment; Authors - Misato Horie ; KO Validated