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ST3GAL5 Polyklonaler Antikörper

ST3GAL5 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30120-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using ST3GAL5 Polyclonal antibody (30120-1-AP)

    WB analysis using 30120-1-AP (same clone as 30120-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30120-1-AP (ST3GAL5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30120-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ST3GAL5 Polyclonal antibody (30120-1-AP)

    IF Staining of U2OS using 30120-1-AP (same clone as 30120-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using ST3GAL5 antibody (30120-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30120-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ST3GAL5 Polyclonal antibody (30120-1-AP)

    IF Staining of U2OS using 30120-1-AP (same clone as 30120-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using ST3GAL5 antibody (30120-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30120-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

30120-1-PBS bindet in WB, IF/ICC, Indirect ELISA ST3GAL5 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ST3GAL5 fusion protein Ag32461
Vollständiger Name ST3 beta-galactoside alpha-2,3-sialyltransferase 5
Berechnetes Molekulargewicht 48 kDa
Beobachtetes Molekulargewicht 70 kDa
GenBank-ZugangsnummerBC065936
Gene symbol ST3GAL5
Gene ID (NCBI) 8869
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ST3GAL5 is also named as SIAT9, GM3 synthase, ST3Gal V, Sialyltransferase 9 and belongs to the glycosyltransferase 29 family. ST3GAL5 transfers the sialyl group (N-acetyl-alpha-neuraminyl or NeuAc) from CMP-NeuAc to the non-reducing terminal galactose of glycosphingolipids forming gangliosides (PMID:9822625, PMID:16934889). SIAT9 is mainly involved in the biosynthesis of ganglioside GM3 but can also use different glycolipids as substrate acceptors such as D-galactosylceramide (GalCer), asialo-GM2 (GA2) and asialo-GM1 (GA1) (PMID:16934889). ST3GAL5 is high expression in brain, skeletal muscle, placenta, and testis. And mRNA of ST3GAL5 is widely distributed in human brain, but slightly elevated expression was observed in the cerebral cortex, temporal lobe, and putamen (PMID:9822625). This antibody recognizes the glycosylated form of ST3GAL5 (PMID: 34943806, PMID: 26458842).