ST3GAL6 Polyklonaler Antikörper

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• KD/KO Validated

ST3GAL6 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13154-1-PBS

Synonyme

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  WB analysis using 13154-1-AP (same clone as 13154-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13154-1-AP (ST3GAL6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13154-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 13154-1-AP (same clone as 13154-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 13154-1-AP (ST3GAL6 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13154-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 13154-1-AP (same clone as 13154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13154-1-AP (ST3GAL6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13154-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human urothelial carcinoma using 13154-1-AP (same clone as 13154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 13154-1-AP (ST3GAL6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13154-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human urothelial carcinoma using 13154-1-AP (same clone as 13154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 13154-1-AP (ST3GAL6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13154-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 13154-1-AP (same clone as 13154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13154-1-AP (ST3GAL6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13154-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13154-1-PBS bindet in WB, IHC, Indirect ELISA ST3GAL6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ST3GAL6 fusion protein Ag3782
• Vollständiger Name: ST3 beta-galactoside alpha-2,3-sialyltransferase 6
• Berechnetes Molekulargewicht: 331 aa, 38 kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: BC023312
• Gene symbol: ST3GAL6
• Gene ID (NCBI): 10402
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ST3GAL6, also named as SIAT10, belongs to the glycosyltransferase 29 family. ST3GAL6 is involved in the synthesis of sialyl-paragloboside, which is a precursor of sialyl-Lewis X determinant. ST3GAL6 has a alpha-2,3-sialyltransferase activity toward Gal-beta1,4-GlcNAc structure on glycoproteins and glycolipids. ST3GAL6 is known to promote cell adhesion and migration of multiple myeloma cells by increasing the synthesis of selectin ligands. ST3GAL6 has 2 isoforms with the molecular mass of 25 and 38 kDa. Sometimes higher molecular weight about 50-60 kDa can also be observed, which may be a glycosylated modified variant of ST3GAL6. (PMID: 31914669, 30220088)